BIOCHEMICAL-CHARACTERIZATION OF CNX NITRATE REDUCTASE-DEFICIENT MUTANTS OF NICOTIANA-PLUMBAGINIFOLIA

被引：7

作者：

MARIONPOLL, A

CHEREL, I

GONNEAU, M

LEYDECKER, MT

机构：

[1] Laboratoire de Biologie Cellulaire, I.N.R.A., F-78026 Versailles Cedex, Route de Saint Cyr

来源：

PLANT SCIENCE | 1991年 / 76卷 / 02期

关键词：

NITRATE REDUCTASE; MOLYBDENUM COFACTOR; MUTANT;

D O I：

10.1016/0168-9452(91)90142-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In cnx mutants of Nicotiana plumbaginifolia, the over-expression of nitrate reductase (NR)-associated NADH cytochrome c reductase (CcR) activity appears to be correlated with increased levels of NR mRNA. However, in ELISA tests, cnxB, C, D, E and F extracts present low levels of cross-reacting material, whereas cnxA extracts exhibit very high levels, indicating an altered NR structure in all cnx mutants except cnxA, due to the absence of a functional molybdenum cofactor (MoCo). Detection of dimeric NR in all cnx mutants, either by gel filtration or native gel electrophoresis, shows that the cofactor is not required for enzyme dimerization, but probably for the stability of the dimer. Similarities between results obtained with cnxB through F mutants, and those nia mutants impaired in the molybdopterin domain, suggest a related NR structure.

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页码：201 / 209

页数：9

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