CONTRACTION OF GUINEA-PIG LUNG PARENCHYMA BY PANCREATIC TYPE PHOSPHOLIPASE-A(2) VIA ITS SPECIFIC BINDING-SITE

被引：55

作者：

KANEMASA, T ^{[1
]}

ARIMURA, A ^{[1
]}

KISHINO, J ^{[1
]}

OHTANI, M ^{[1
]}

ARITA, H ^{[1
]}

机构：

[1] SHIONOGI & CO LTD,SHIONOGI RES LAB,12-4 SAGISU 5-CHOME,FUKUSHIMA KU,OSAKA 553,JAPAN

来源：

FEBS LETTERS | 1992年 / 303卷 / 2-3期

关键词：

PHOSPHOLIPASE-A(2); SPECIFIC BINDING SITE; THROMBOXANE-A(2); GUINEA PIG LUNG PARENCHYMA; CONTRACTION; (+)-S-145NA;

D O I：

10.1016/0014-5793(92)80523-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porcine pancreatic group I phospholipase A2 (PLA2-I) induced contraction of guinea pig lung parenchyma in a concentration-dependent manner. Its EC50 value was similar to the K(d) value calculated from the specific binding of I-125-labeled porcine PLA2-I in the membrane fraction of guinea pig lung. Type-specific action of PLA2's and homologous desensitization strongly implicated the involvement of PLA2-I-specific sites in the activation process. Thromboxane A2 was found to be the main product from lung tissue by PLA2-I action and the contractile response by PLA2-I was specifically suppressed by thromboxane A2 receptor antagonists and cyclooxygenase inhibitor, but not by leukotriene receptor antagonist and H1 blocker. These findings indicate that PLA2-I-induced contractile response may depend on the secondarily produced thromboxane A2, thus providing a new aspect of PLA2-I from the pathophysiological standpoint.

引用

页码：217 / 220

页数：4

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