MULTIPLE RELEASABLE FLUORESCEIN LABELS FOR IMMUNOASSAY - THE PRINCIPLE ILLUSTRATED BY AN IMMUNOASSAY FOR ANTIBODIES TO THE HUMAN-IMMUNODEFICIENCY-VIRUS

被引：4

作者：

BERTRAM, VM ^{[1
]}

BAILEY, MP ^{[1
]}

ROCKS, BF ^{[1
]}

机构：

[1] ROYAL SUSSEX CTY HOSP,DEPT BIOCHEM,EASTERN RD,BRIGHTON BN2 5BE,E SUSSEX,ENGLAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1991年 / 28卷

关键词：

AIDS; DISULFIDE LINK; MICROTITRATION PLATE;

D O I：

10.1177/000456329102800511

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Attempts to increase the sensitivity of fluorescein-based fluorescence immunoassays by using multiple labelling have generally been unsuccessful because of concentration quenching. We have labelled antibodies to human immunoglobulin G with multiple fluorescein fluorophores attached by means of a disulphide linkage: this linkage can be rapidly and easily broken by treatment with dithiothreitol, allowing fluorescein to be released from the antibody and measured in free solution. Application of this technique to a fluorescence labelled immunosorbent assay for antibodies to the human immunodeficiency virus gave an approximately 20-fold increase in signal compared with an equivalent assay using fluorescein isothiocyanate.

引用

页码：487 / 491

页数：5

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