QUANTITATION OF CARBOHYDRATE SWEETENERS AND ORGANIC-ACIDS IN HUMAN ORAL FLUID USING HPLC ANALYSIS

被引：10

作者：

LINKE, HAB

MOSS, SJ

机构：

[1] Departments of Microbiology and Pediatric Dentistry, New York University Dental Center

来源：

ZEITSCHRIFT FUR ERNAHRUNGSWISSENSCHAFT | 1992年 / 31卷 / 02期

关键词：

SUGARS; CARBOHYDRATES; LACTIC ACID; ORAL FLUID; HPLC; GLUCOSE; SUCROSE;

D O I：

10.1007/BF01623073

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

A sensitive high performance liquid chromatography (HPLC) assay was developed for the qualitative and quantitative determination of carbohydrate sweeteners and organic acids in oral fluid. To separate these compounds, an ion-moderated partition resin HPLC column (Aminex HPX-87H) was used. All components of the HPLC system were interconnected using stainless steel capillary tubing. Isocratic elution with 0.01 N sulfuric acid provided the profile of both compound classes. The compounds were detected using a refractive index detector. The method employed computerized data collection and integration (Omega-2 system) with a detection sensitivity of 0.1-mu-g compound per HPLC assay (80-mu-l). This method is useful in caries research, because it detects minute amounts of sugars and organic acids in oral fluid during clearance studies of various foods in the mouth.

引用

页码：147 / 154

页数：8

共 8 条

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