APPLICATION OF MAGNETIC-SECTOR THERMAL IONIZATION MASS-SPECTROMETRY TO STUDIES OF ERYTHROCYTE IRON INCORPORATION IN SMALL CHILDREN

被引：25

作者：

ABRAMS, SA ^{[1
]}

WEN, JP ^{[1
]}

OBRIEN, KO ^{[1
]}

STUFF, JE ^{[1
]}

LIANG, LK ^{[1
]}

机构：

[1] TEXAS CHILDRENS HOSP,HOUSTON,TX 77030

来源：

BIOLOGICAL MASS SPECTROMETRY | 1994年 / 23卷 / 12期

关键词：

D O I：

10.1002/bms.1200231209

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The optimal evaluation of iron metabolism requires the administration of two isotopes of iron, However, high-precision measurement of isotopic ratios from blood samples obtained after administration of two stable isotopes of iron to human subjects has not previously been reported, Using a cation-exchange system to isolate iron from blood samples, we found that high-precision (<0.2%) measurements of Fe-58/Fe-56 and Fe-57/Fe-56 could be performed using magnetic sector thermal ionization mass spectrometry. Clinical studies in four 1-year-old infants showed that this technique could be used to demonstrate a lower rate of iron absorption in small children given an iron supplement (Fe-57) with milk compared to those given iron (Fe-58 and ferrous sulfate) with ascorbic acid, This technique will enable the evaluation of iron metabolism in populations in whom the use of radioactive iron tracers is not appropriate.

引用

页码：771 / 775

页数：5

共 14 条

[1] CLINICAL-STUDIES OF MINERAL METABOLISM IN CHILDREN USING STABLE ISOTOPES [J].