DIFFERENTIAL SCANNING CALORIMETRY OF LOBSTER HEMOCYANIN

被引：58

作者：

GUZMANCASADO, M ^{[1
]}

PARODYMORREALE, A ^{[1
]}

MATEO, PL ^{[1
]}

SANCHEZRUIZ, JM ^{[1
]}

机构：

[1] UNIV GRANADA,FAC CIENCIAS,DEPT QUIM FIS,E-18071 GRANADA,SPAIN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 188卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15386.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential scanning calorimetry has been performed with Palinurus vulgaris haemocyanin monomers and hexamers. The denaturation of the protein is irreversible. Both the temperature of the transition maximum and the enthalpy are lower for the monomer than for the hexamer. A scan rate dependence of the temperature of the maxims if found for both the monomer and the hexamer; for the hexamer at least, this can be explained in terms of a two‐state kinetic model. Some comments are made as to the use of equilibrium thermodynamics in the analysis of irreversible scanning calorimetric traces. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：181 / 185

页数：5

共 28 条

[1] MOLECULAR-WEIGHTS OF PROTEIN MULTIMERS FROM POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

BRYAN, JK .

ANALYTICAL BIOCHEMISTRY, 1977, 78 (02) :513-519

[2] DIFFERENTIAL SCANNING CALORIMETRIC STUDY OF THE THERMAL-DENATURATION OF ASPARTATE TRANSCARBAMOYLASE OF ESCHERICHIA-COLI [J].

EDGE, V ;

ALLEWELL, NM ;

STURTEVANT, JM .

BIOCHEMISTRY, 1988, 27 (21) :8081-8087

[3] HIGH-RESOLUTION DIFFERENTIAL SCANNING CALORIMETRIC ANALYSIS OF THE SUBUNITS OF ESCHERICHIA-COLI ASPARTATE TRANSCARBAMOYLASE [J].

EDGE, V ;

ALLEWELL, NM ;

STURTEVANT, JM .

BIOCHEMISTRY, 1985, 24 (21) :5899-5906

[4] STARCH-GEL ELECTROPHORESIS-APPLICATION TO CLASSIFICATION OF PITUITARY PROTEINS + POLYPEPTIDES [J].

FERGUSON, KA .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1964, 13 (10P) :985-+

[5]

FUKADA H, 1983, J BIOL CHEM, V258, P3193

[6] 3.2 A STRUCTURE OF THE COPPER-CONTAINING, OXYGEN-CARRYING PROTEIN PANULIRUS-INTERRUPTUS HEMOCYANIN [J].

GAYKEMA, WPJ ;

HOL, WGJ ;

VEREIJKEN, JM ;

SOETER, NM ;

BAK, HJ ;

BEINTEMA, JJ .

NATURE, 1984, 309 (5963) :23-29

[7] CHEMICAL STUDIES ON HEMOCYANINS .I. AMINO ACID COMPOSITION [J].

GHIRETTIMAGALDI, A ;

NUZZOLO, C ;

GHIRETTI, F .

BIOCHEMISTRY, 1966, 5 (06) :1943-+

[8] SCANNING CALORIMETRIC STUDY OF THE THERMAL UNFOLDING OF CATABOLITE ACTIVATOR PROTEIN FROM ESCHERICHIA-COLI IN THE ABSENCE AND PRESENCE OF CYCLIC MONONUCLEOTIDES [J].

GHOSAINI, LR ;

BROWN, AM ;

STURTEVANT, JM .

BIOCHEMISTRY, 1988, 27 (14) :5257-5261

[9] GENERALIZED BINDING PHENOMENA IN AN ALLOSTERIC MACROMOLECULE [J].

GILL, SJ ;

RICHEY, B ;

BISHOP, G ;

WYMAN, J .

BIOPHYSICAL CHEMISTRY, 1985, 21 (01) :1-14

[10] THERMODYNAMIC STUDY OF YEAST PHOSPHOGLYCERATE KINASE [J].

HU, CQ ;

STURTEVANT, JM .

BIOCHEMISTRY, 1987, 26 (01) :178-182

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