ANTISENSE RNA EFFICIENTLY INHIBITS FORMATION OF THE 10 KD POLYPEPTIDE OF PHOTOSYSTEM-II IN TRANSGENIC POTATO PLANTS - ANALYSIS OF THE ROLE OF THE 10 KD PROTEIN

被引:61
作者
STOCKHAUS, J
HOFER, M
RENGER, G
WESTHOFF, P
WYDRZYNSKI, T
WILLMITZER, L
机构
[1] UNIV DUSSELDORF,INST ENTWICKLUNGS & MOLEK BIOL PFLANZEN,W-4000 DUSSELDORF 1,GERMANY
[2] TECH UNIV BERLIN,MAX VOLLMER INST PHYS & BIOPHYS CHEM,W-1000 BERLIN 61,GERMANY
关键词
10 kd protein; anti-sense RNA; photosystem II; transgenic plants;
D O I
10.1002/j.1460-2075.1990.tb07494.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A chimeric gene encoding an anti-sense RNA of the 10 kd protein of the water-splitting apparatus of photosystem II of higher plants under the control of the CaMV 35S promoter was introduced into potato using Agrobacterium based vectors. The expression of the anti-sense RNA led to a significant reduction of the amounts of the 10 kd protein and RNA in a number of transgenic plants. In three out of 36 plants tested, the level of the 10 kd protein was only up to 1-3% compared with the wild-type control. The drastic reduction of the 10 kd protein did not influence the accumulation of other photosystem II associated polypeptides at both the RNA and protein level. Furthermore no phenotypic differences were observed between potato plants expressing wild-type and drastically reduced the levels of the 10 kd protein with respect to growth rate, habitus or ultrastructure of the chloroplasts. Measurements of the relaxtion of the flash-induced enhancement in the fluorescence quantum yield as determined in intact leaves and the rates and characteristic oscillation pattern of O2 evolution as determined in isolated thylakoid samples however, show that the elimination of the 10 kd protein on the one hand retards reoxidation of Q(A) - and on the other hand introduces a general disorder into the PSII complex.
引用
收藏
页码:3013 / 3021
页数:9
相关论文
共 54 条
  • [1] RECONSTITUTION OF PHOTOSYNTHETIC WATER SPLITTING IN INSIDE-OUT THYLAKOID VESICLES AND IDENTIFICATION OF A PARTICIPATING POLYPEPTIDE
    AKERLUND, HE
    JANSSON, C
    ANDERSSON, B
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 681 (01) : 1 - 10
  • [2] ELECTRON-TRANSPORT IN PHOTOSYSTEM-I AND PHOTOSYSTEM-II
    ANDREASSON, LE
    VANNGARD, T
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1988, 39 : 379 - 411
  • [3] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS
    ARNON, DI
    [J]. PLANT PHYSIOLOGY, 1949, 24 (01) : 1 - 15
  • [4] THE IMPLICATION OF A PLASTID-DERIVED FACTOR IN THE TRANSCRIPTIONAL CONTROL OF NUCLEAR GENES ENCODING THE LIGHT-HARVESTING CHLOROPHYLL A-B PROTEIN
    BATSCHAUER, A
    MOSINGER, E
    KREUZ, K
    DORR, I
    APEL, K
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 154 (03): : 625 - 634
  • [5] BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION
    BEVAN, M
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (22) : 8711 - 8721
  • [6] CYTOPLASMIC SYNTHESIS OF PLASTID POLYPEPTIDES MAY BE CONTROLLED BY PLASTID-SYNTHESIZED RNA
    BRADBEER, JW
    ATKINSON, YE
    BORNER, T
    HAGEMANN, R
    [J]. NATURE, 1979, 279 (5716) : 816 - 817
  • [7] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [8] MECHANISM OF PHOTOSYNTHETIC WATER OXIDATION
    BRUDVIG, GW
    BECK, WF
    DEPAULA, JC
    [J]. ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY, 1989, 18 : 25 - 46
  • [9] DEBLAERE R, 1985, NUCLEIC ACIDS RES, V13, P4777, DOI 10.1093/nar/13.13.4777
  • [10] THE STRUCTURAL BASIS OF PHOTOSYNTHETIC LIGHT REACTIONS IN BACTERIA
    DEISENHOFER, J
    MICHEL, H
    HUBER, R
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (06) : 243 - 248