DETERMINATION OF MALONDIALDEHYDE WITH CHEMICAL DERIVATIZATION INTO THE PYRMIDINE COMPOUND AND HPLC

被引：22

作者：

KISHIDA, E

ORIBE, M

MOCHIZUKI, K

KOJO, S

IGUCHI, H

机构：

[1] HYOGO UNIV TEACHERS EDUC,DEPT LIFE & HLTH SCI,HYOGO 67314,JAPAN

[2] KYOTO UNIV,FAC MED,DEPT PUBL HLTH,KYOTO 606,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1045卷 / 02期

关键词：

2-Thiobarbituric acid reaction; Lipid peroxidation; Malondialdehyde;

D O I：

10.1016/0005-2760(90)90149-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new HPLC method to determine malondialdehyde (MDA) was developed. Malondialdehyde was reacted with α-N-benzoyl-l-arginine ethyl ester and converted into α-N-benzoyl-δ-N-(2-pyrimidinyl)-l-ornithine ethyl ester, which is sufficiently hydrophobic to allow us its specific determination utilizing a reversed-phase C18 column at the level of 1 pmol. © 1990.

引用

页码：187 / 188

页数：2