EFFECTS OF OXYGEN AERATION ON PRODUCTION OF MONOCLONAL-ANTIBODY IN IMMOBILIZED HYBRIDOMA-CELL BIOREACTOR

被引：12

作者：

KAMIHIRA, M ^{[1
]}

YOSHIDA, H ^{[1
]}

IIJIMA, S ^{[1
]}

KOBAYASHI, T ^{[1
]}

机构：

[1] NAGOYA UNIV,FAC ENGN,DEPT CHEM ENGN,CHIKUSA KU,NAGOYA 46401,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 69卷 / 05期

关键词：

D O I：

10.1016/0922-338X(90)90113-B

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d. © 1989.

引用

页码：311 / 312

页数：2

共 8 条

[1] TECHNIQUES FOR MAMMALIAN-CELL IMMOBILIZATION [J].