EXTRACELLULAR PRODUCTION OF L-LYSINE ALPHA-OXIDASE IN WHEAT BRAN CULTURE OF A STRAIN OF TRICHODERMA-VIRIDE

A mold strain Y244 2 capable of producing L-Iysine a-oxidase, a new enzyme catalyzing the a-oxidative deamination of L-lysine, was identified as Trichoderma viride. Among strains belonging to the genus Trichoderma tested, only Trichoderma viride Y244--2 produced the enzyme in wheat bran culture. The maximum enzyme production by the mold grown on wheat bran was observed after 10 and 14 days incubation with and without NaNO3, respectively. Addition of NaNO3, NH4NO3, adenine, purine nucleosides, L-histidine, glycine or L-glutamine to wheat bran stimulated the production of the enzyme. In the liquid culture, the enzyme was produced extracellulary under the aerobic conditions, although the production was much lower than that in the wheat bran culture. © 1979 Taylor & Francis Group, LLC.