HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF HYDROXYCHLOROQUINE AND ITS MAJOR METABOLITES IN BIOLOGICAL-FLUIDS USING AN ALPHA-1-ACID GLYCOPROTEIN STATIONARY PHASE

被引：37

作者：

MCLACHLAN, AJ ^{[1
]}

TETT, SE ^{[1
]}

CUTLER, DJ ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT PHARM,SYDNEY,NSW 2006,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 570卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80206-R

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enantioselective two-stage off-line assay has been developed for the analysis of hydroxychloroquine and its three major metabolites in biological fluids. The first non-stereoselective stage of the assay (PRP-1 column) separates and quantitates parent drug and metabolites. Fractions containing hydroxychloroquine and each of the metabolites are collected manually, evaporated, reconstituted in mobile phase and re-injected onto an alpha-1-acid glycoprotein column to separate and determine proportions of individual enantiomers. Preliminary results from patient samples indicate that the disposition of hydroxychloroquine and its major metabolites is enantioselective.

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页码：119 / 127

页数：9

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