学术探索
学术期刊
新闻热点
数据分析
智能评审

THE INTERACTION OF BENZHYDROXAMIC ACID WITH HORSERADISH-PEROXIDASE AND ITS FLUORESCENT ANALOGS

被引：36
作者：
AVIRAM, I
机构：
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1981年 / 212卷 / 02期
关键词：
D O I：
10.1016/0003-9861(81)90391-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Horseradish peroxidase (HRP) reconstituted with protoporphyrin IX or zinc protoporphyrin IX binds benzhydroxamic acid with affinities of 1.54 .times. 104 and 8 .times. 102 M-1, respectively. This interaction is competitive with respect to hydrogen donor substrates of peroxidase. The steady-state oxidation of benzhydroxamic acid by HRP was studied by monitoring the disappearance of the hydroxamate function and the formation of nitrite. The inhibition by benzhydroxamic acid of oxidation of HRP substrates may be classified as an inhibition by a competing substrate. In the each of HRP-catalyzed oxidation of ferrocyanide a marked activating effect of benzhydroxamic acid was observed. Mechanisms responsible for this effect are discussed.
引用
收藏
页码：483 / 490
页数：8
相关论文
共 26 条
[1]   CHEMISTRY OF HYDROXAMIC ACIDS AND N-HYDROXYIMIDES [J].
BAUER, L ;
EXNER, O .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1974, 13 (06) :376-384
[2]   THE KINETICS AND STOICHIOMETRY OF THE TRANSITION FROM THE PRIMARY TO THE SECONDARY PEROXIDASE PEROXIDE COMPLEXES [J].
CHANCE, B .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1952, 41 (02) :416-424
[3]  
CORMIER MJ, 1968, J BIOL CHEM, V243, P4706
[4]  
CRITCHLOW JE, 1972, J BIOL CHEM, V247, P3703
[5]  
DUNFORD HB, 1975, J BIOL CHEM, V250, P2920
[6]   FUNCTION AND MECHANISM OF ACTION OF PEROXIDASES [J].
DUNFORD, HB ;
STILLMAN, JS .
COORDINATION CHEMISTRY REVIEWS, 1976, 19 (03) :187-251
[7]   THE CHEMICAL NATURE OF THE 2ND HYDROGEN PEROXIDE COMPOUND FORMED BY CYTOCHROME-C PEROXIDASE AND HORSERADISH PEROXIDASE .1. TITRATION WITH REDUCING AGENTS [J].
GEORGE, P .
BIOCHEMICAL JOURNAL, 1953, 54 (02) :267-276
[8]   WATER PROTON RELAXATION STUDIES OF THE HEME-ENVIRONMENT IN MN(III)-SUBSTITUTED AND NATIVE HORSERADISH PEROXIDASES [J].
GUPTA, RK ;
MILDVAN, AS ;
SCHONBAUM, GR .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1980, 202 (01) :1-7
[9]  
HASINOFF BB, 1970, BIOCHEMISTRY-US, V9, P4930
[10]   COMPARISONS BETWEEN HOG INTESTINAL PEROXIDASE AND BOVINE LACTOPEROXIDASE COMPOUND-I FORMATION AND INHIBITION BY BENZHYDROXAMIC ACID [J].
KIMURA, S ;
YAMAZAKI, I .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1979, 198 (02) :580-588
123