CLONING AND EXPRESSION OF A CDNA FOR RAT PROSTACYCLIN RECEPTOR

被引：39

作者：

SASAKI, Y

USUI, T

TANAKA, I

NAKAGAWA, O

SANDO, T

TAKAHASHI, T

NAMBA, T

NARUMIYA, S

NAKAO, K

机构：

[1] DEPT MED,DIV 2,SAKYO KU,KYOTO 606,JAPAN

[2] KYOTO UNIV,FAC MED,DEPT PHARMACOL,SAKYO KU,KYOTO 606,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1224卷 / 03期

关键词：

PROSTACYCLIN (PGI(2)); RECEPTOR; CLONING;

D O I：

10.1016/0167-4889(94)90300-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone for rat prostacyclin receptor was isolated. The cDNA encodes a protein of 416 amino acid residues (M(r) 44662) with putative seven transmembrane domains, and belongs to the G protein-coupled receptor superfamily. Specific binding of [H-3]iloprost was found in membrane of COS-7 cells transfected with the cDNA (K-d = 1.3 nM) and was displaced with unlabeled prostaglandins in the order of iloprost = cicaprost > PGE(1) > STA(2) = PGE(2) = PGD(2) > PGF(2 alpha). Northern blot analysis demonstrated that rat prostacyclin receptor mRNA is expressed in the lung, spleen, heart, pancreas, thymus, stomach and aorta.

引用

页码：601 / 605

页数：5

共 18 条

[1] FUNCTIONAL AND LIGAND-BINDING STUDIES SUGGEST HETEROGENEITY OF PLATELET PROSTACYCLIN RECEPTORS [J].