DNA-BINDING FLUOROCHROMES FOR STUDY OF ORGANIZATION OF METAPHASE NUCLEUS

被引：228

作者：

CASPERSSON, T

ZECH, L

MODEST, EJ

FOLEY, GE

WAGH, U

SIMONSSON, E

机构：

[1] Institute for Medical Cell Research and Genetics, Karolinska Institutet, Stockholm

[2] The Children's Cancer Research Foundation, Department of Pathology, Harvard Medical School, Boston

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 58卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0014-4827(69)90124-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Fluorescent DNA-reagents with different modes of binding have been used for the purpose of identifying chemical differences along metaphase chromosomes. The DNA-distribution pattern along the chromosomes, determined with very high resolution procedures, has been used as reference when studying the differential uptake of different chromosome regions of the compounds. For quantitative measurements of the fluorescence of very small objects a special system has been developed. Several types of plant material and also Chinese hamster have been investigated. Great and reproducible differences in the uptake of the fluorescent reagents by different chromosome regions were observed. In Trillium a good correlation was found between regions reacting with several different fluorescent compounds and heterochromatin as defined by cold treatment. The looseness of the several presently used definitions of heterochromatin is stressed. © 1969.

引用

页码：141 / +

页数：1

共 20 条

[1] DIFFERENTIAL INTERACTION OF NOGALAMYCIN WITH DNA OF VARYING BASE COMPOSITION [J].