NEUROCHEMICAL EVIDENCE FOR THE INVOLVEMENT OF N-TYPE CALCIUM CHANNELS IN TRANSMITTER SECRETION FROM PERIPHERAL ENDINGS OF SENSORY NERVES IN GUINEA-PIGS

被引：96

作者：

MAGGI, CA ^{[1
]}

TRAMONTANA, M ^{[1
]}

CECCONI, R ^{[1
]}

SANTICIOLI, P ^{[1
]}

机构：

[1] UNIV FLORENCE,INST PHARMACOL,I-50121 FLORENCE,ITALY

来源：

NEUROSCIENCE LETTERS | 1990年 / 114卷 / 02期

关键词：

Capsaicin-sensitive primary afferent; Electrical field stimulation; Sensory neuropeptide; ω-Conotoxin;

D O I：

10.1016/0304-3940(90)90072-H

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In the guinea pig ureter, substance P- (SP) and calcitonin gene-related peptide- (CGRP) like immunoreactivity (LI) were depleted by systemic capsaicin pretreatment, indicating that they are entirely stored in peripheral endings of primary afferent neurones. Electrical field stimulation (20 Hz, 60 V, 0.5 ms) evoked the simultaneous release of SP- and CGRP-LI from superfused guinea pig ureters which was abolished by tetrodotoxin (0.3 μM). ω-Conotoxin (0.1 μM), a potent blocker of N-type voltage-sensitive calcium channels, reduced by 50-70% the evoked release of both peptides. These findings provide direct neurochemical evidence indicating that conotoxin-sensitive calcium channels play a role in transmitter secretion evoked by antidromic invasion of peripheral terminals of capsaicin-sensitive primary afferents. © 1990.

引用

页码：203 / 206

页数：4

共 15 条

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