INDUCTION BY EGF AND INTERFERON-GAMMA OF TYROSINE-PHOSPHORYLATED DNA-BINDING PROTEINS IN MOUSE-LIVER NUCLEI

被引：296

作者：

RUFFJAMISON, S ^{[1
]}

CHEN, K ^{[1
]}

COHEN, S ^{[1
]}

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT BIOCHEM,NASHVILLE,TN 37232

来源：

SCIENCE | 1993年 / 261卷 / 5129期

关键词：

D O I：

10.1126/science.8378774

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intraperitoneal injection of epidermal growth factor (EGF) into mice resulted in the appearance in liver nuclei of three tyrosine phosphorylated proteins (84, 91, and 92 kilodaltons) within minutes after administration of EGF. Administration of interferon-gamma (IFN-gamma) resulted in the appearance in liver nuclei of two tyrosine phosphorylated proteins (84 and 91 kilodaltons). The 84- and 91-kilodalton proteins detected after either EGF or IFN-gamma administration were identified as the IFN-gamma activation factors (GAF). Furthermore, gel shift analysis revealed that these GAF proteins, detected after either EGF or IFN-gamma administration, specifically bound to the sis-inducible element of the c-fos promoter. Thus, GAF proteins participate in nuclear signaling in both IFN-gamma and EGF pathways.

引用

页码：1733 / 1736

页数：4

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