2 NEW MULTIPURPOSE MULTICOPY SCHIZOSACCHAROMYCES-POMBE SHUTTLE VECTORS, PSP1 AND PSP2

被引：85

作者：

COTTAREL, G

BEACH, D

DEUSCHLE, U

机构：

[1] Cold Spring Harbor Laboratory, Howard Hughes Medical Institute, Cold Spring Harbor, 11724, NY

来源：

CURRENT GENETICS | 1993年 / 23卷 / 5-6期

关键词：

AUTONOMOUSLY REPLICATING SEQUENCE; RECOMBINANT DNA; NESTED DELETIONS; MUTAGENESIS; SCHIZOSACCHAROMYCES-POMBE;

D O I：

10.1007/BF00312650

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.

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页码：547 / 548

页数：2

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