Scientific Opinion on VTEC-seropathotype and scientific criteria regarding pathogenicity assessment

被引：142

作者：

Andreoletti, Olivier ^{[1
]}

Baggesen, Dorte Lau ^{[1
]}

Bolton, Declan ^{[1
]}

Butaye, Patrick ^{[1
]}

Cook, Paul ^{[1
]}

Davies, Robert ^{[1
]}

Escamez, Pablo S. Fernandez ^{[1
]}

Griffin, John ^{[1
]}

Hald, Tine ^{[1
]}

Havelaar, Arie ^{[1
]}

Koutsoumanis, Kostas ^{[1
]}

Lindqvist, Roland ^{[1
]}

McLauchlin, James ^{[1
]}

Nesbakken, Truls ^{[1
]}

Maradona, Miguel Prieto ^{[1
]}

Ricci, Antonia ^{[1
]}

Ru, Giuseppe ^{[1
]}

Sanaa, Moez ^{[1
]}

Simmons, Marion ^{[1
]}

Sofos, John ^{[1
]}

Threlfall, John ^{[1
]}

机构：

[1] EFSA, Parma, Italy

来源：

EFSA JOURNAL | 2013年 / 11卷 / 04期

关键词：

VTEC; virulence factors; serogroup; seropathotype; detection; isolation; identification;

D O I：

10.2903/j.efsa.2013.3138

中图分类号：

TS2 [食品工业];

学科分类号：

0832 [食品科学与工程];

摘要：

During 2007-2010, 13 545 confirmed human VTEC infections and 777 haemolytic uraemic syndrome (HUS) cases were reported in the EU; isolates from 85 % of cases were not fully serotyped and therefore could not be classified using the Karmali seropathotype concept. Seropathotype group D covered 5 % of isolates from fully serotyped cases; 14 cases (0.7 %) belonged to seropathotype group E, defined by Karmali et al. (2003) as nonhuman only. Isolates from around 27 % of cases could not be assigned. There were no HUS cases reported for the serotypes in groups D and E but 17 HUS cases could not be assigned. The health outcome was reported for only a fraction of confirmed cases. About 64 % of patients presented with only diarrhoea; VTEC infection resulted in HUS in around 10 % of cases. The new ISO/TS 13136: 2012 standard improves the detection of VTEC in food. An alternative concept based on the detection of verocytotoxins alone or genes encoding such verocytotoxins does not provide a sound scientific basis on which to assess risk to the consumer because there is no single or combination of marker(s) that fully define a, pathogenic. VTEC. Strains positive for verocytotoxin 2 gene (vtx2)-and eae (intimin production)-or [aaiC (secreted protein of EAEC) plus aggR (plasmid-encoded regulator)] genes are associated with higher risk of more severe illness than other virulence gene combinations. The 2011 O104: H4 outbreak demonstrated the difficulty of predicting the emergence of, new. pathogenic VTEC types by screening only for the eae gene or by focusing on a restricted panel of serogroups. A molecular approach utilising genes encoding virulence characteristics additional to the presence of vtx genes has been proposed. (C) European Food Safety Authority, 2013.

引用

页数：106

共 117 条

[1]

Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments [J].