2-MUM COVALENTLY CLOSED NON-MITOCHONDRIAL CIRCULAR DNA IN THE PETITE-NEGATIVE YEAST SCHIZOSACCHAROMYCES-POMBE

被引:19
作者
DELGIUDICE, L [1 ]
WOLF, K [1 ]
SASSONECORSI, P [1 ]
MAZZA, A [1 ]
机构
[1] UNIV MUNICH,INST GENET,D-8000 MUNICH 22,FED REP GER
来源
MOLECULAR & GENERAL GENETICS | 1979年 / 172卷 / 02期
关键词
D O I
10.1007/BF00268278
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A population of small covalently closed non-mitochondrial circular DNA molecules was isolated from the petite-negative yeast Schizosaccharomyces pombe. The mean length of these molecules, possessing the same density as nuclear DNA (1.695 g/ cm3) is 1.95±0.18 μm. The presence of these minicircles in crude mitochondrial preparations indicates their tight association with mitochondrial particles. Their disappearance after DNase treatment of mitochondria demonstrates their extramitochondrial location. © 1979 Springer-Verlag.
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页码:165 / 169
页数:5
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