THE DEVELOPMENT OF DRUG-METABOLIZING-ENZYMES IN FEMALE SHEEP LIVERS

被引:24
作者
KADDOURI, M [1 ]
LARRIEU, G [1 ]
EECKHOUTTE, C [1 ]
GALTIER, P [1 ]
机构
[1] INRA,PHARMACOL TOXICOL LAB,180 CHEM TOURNEFEUILLE,F-31931 TOULOUSE,FRANCE
关键词
D O I
10.1111/j.1365-2885.1990.tb00787.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Kaddouri, M., Larrieu, G., Eeckhoutte, C. & Galtier, P. The development of drug‐metabolizing enzymes in female sheep livers. J. vet. Pharmacol. Therap. 13, 340–349. The purpose of this investigation was to determine age‐related changes of some hepatic drug‐metabolizing activities in Lacaune ewes in the foetal, neonatal (1 and 4 weeks), growing (7 months), pregnant (11 months) and adult (6 years) stages. Although microsomal cytochrome P‐450 was not detected in 3‐month‐old foetuses, it increased regularly from 1‐week‐to 11‐month‐old animals. Among mixed‐function oxidases, the development of aminopyrine and ethylmorphine N‐demethylases, benzo(α)pyrene hydroxylase and ethoxycoumarin O‐deethylase were correlated to that of total cytochrome P‐450. Due to their presence in foetal liver or their more rapid evolution, cytochrome b5, NADPH cytochrone c reductase, aniline hydroxylase, benzphetamine N‐demethylase and erythromycin N‐demethylase did not parallel the ontogenesis of cytochrome P‐450. Hepatic transferases showed different developmental patterns from mono‐oxygenases, so UDP glucuronyltransferase was detected in the foetus, reached maximum activity in all young ages up to the pregnant stage and subsequently fell in adult ewes. Concerning glutathione S‐transferase accepting l‐chloro‐2,4‐dinitrobenzene as substrate, similar values were obtained in the foetus and all young animals, whereas five‐to tenfold higher values were obtained in both pregnant and adult female sheep. N‐acetyltransferase using sulphamethazine did not significantly change from foetuses to adults but there were large differences in the capacity of hepatic acetylation between animals belonging to the same group. P. Galtier, Laboratoire de Pharmacologie‐Toxicologie, INRA, 180 chemin de Tournefeuille, BP 3, 31931 Toulouse, France. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:340 / 349
页数:10
相关论文
共 27 条
[1]  
AITO A, 1978, ANAL BIOCHEM, V85, P488
[2]   CHARACTERIZATION OF SHEEP LIVER AND LUNG MICROSOMAL ETHYLMORPHINE N-DEMETHYLASE [J].
ARINC, E .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1985, 80 (02) :389-399
[3]   EFFECT OF RUMEN DEVELOPMENT AND PREEXPOSURE TO CHEMICALS ON THE ACTIVITY OF THE MIXED-FUNCTION OXIDASE SYSTEM IN GOATS [J].
BURLEY, FE ;
BRAY, TM .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1983, 75 (01) :137-140
[4]  
COCHIN J, 1959, J PHARMACOL EXP THER, V125, P105
[5]   REGULATION OF DRUG-METABOLIZING-ENZYMES DURING THE PERINATAL-PERIOD IN RAT AND HUMAN-LIVER [J].
CRESTEIL, T .
BIOESSAYS, 1987, 7 (03) :120-124
[6]  
CRESTEIL T, 1986, J PHARMACOL EXP THER, V236, P269
[7]   HEPATIC CYTOCHROME-P-450 DEPENDENT DRUG-METABOLIZING ACTIVITY IN RATS, RABBITS AND SEVERAL FOOD-PRODUCING SPECIES [J].
DALVI, RR ;
NUNN, VA ;
JUSKEVICH, J .
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 1987, 10 (02) :164-168
[8]   HYDROXYLATION AND GLUCURONIDATION OF VARIOUS XENOBIOTICS BY HEPATIC MICROSOMES FROM THE FETAL LAMB, PREGNANT EWE AND HUMAN-FETUS [J].
DVORCHIK, BH ;
WOODWARD, G ;
SITAR, DS ;
TWEED, WA .
DEVELOPMENTAL PHARMACOLOGY AND THERAPEUTICS, 1986, 9 (04) :282-289
[10]  
GALTIER P, 1986, DRUG METAB DISPOS, V14, P137