GLUTAMINE-SYNTHETASE IN OLIGODENDROCYTES AND ASTROCYTES - NEW BIOCHEMICAL AND IMMUNOCYTOCHEMICAL EVIDENCE

被引：113

作者：

TANSEY, FA ^{[1
]}

FAROOQ, M ^{[1
]}

CAMMER, W ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT NEUROL,F-140,1300 MORRIS PK AVE,BRONX,NY 10461

来源：

JOURNAL OF NEUROCHEMISTRY | 1991年 / 56卷 / 01期

关键词：

GLUTAMINE SYNTHETASE; OLIGODENDROCYTES; ASTROCYTES; GLIAL CELLS; BOVINE BRAIN; RAT BRAIN;

D O I：

10.1111/j.1471-4159.1991.tb02591.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The results of recent immunocytochemical experiments suggest that glutamine synthetase (GS) in the rat CNS may not be confined to astrocytes. In the present study, GS activity was assayed in oligodendrocytes isolated from bovine brain and in oligodendrocytes, astrocytes, and neurons isolated from rat forebrain, and the results were compared with new immunochemical data. Among the cells isolated from rat brain, astrocytes had the highest specific activities of GS, followed by oligodendrocytes. Oligodendrocytes isolated from white matter bovine brain had GS specific activities almost fivefold higher than those in white matter homogenates. Immunocytochemical staining also showed the presence of GS in both oligodendrocytes and astrocytes in bovine forebrain, in three white-matter regions of rat brain, and in Vibratome sections as well as paraffin sections.

引用

页码：266 / 272

页数：7

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