CHARACTERISTIC ELECTRON-MICROSCOPIC PROJECTIONS OF THE SMALL RIBOSOMAL-SUBUNIT FROM THERMOMYCES-LANUGINOSUS

被引：10

作者：

HARAUZ, G

FLANNIGAN, D

机构：

[1] Department of Molecular Biology and Genetics, University of Guelph, Guelph

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1130卷 / 03期

关键词：

RIBOSOME; THERMOPHILE; FUNGUS; ELECTRON MICROSCOPY; IMAGE ANALYSIS;

D O I：

10.1016/0167-4781(92)90441-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multivariate statistical analysis and hierarchical ascendant classification techniques have been used to sort electron images of small ribosomal subunits from the thermophilic fungus Thermomyces lanuginosus into their characteristic views. Three predominant modes of adsorption to the support were elucidated: right-lateral, left-lateral and asymmetric, showing reproducible detail approaching 1.8 nm resolution. The projections of the fungal complexes appeared almost identical to those of HeLa cells, rat liver and rabbit reticulocytes studied previously in this manner. This result contrasts with the greater variation in fine structural features observed between ribosomal subunits from different prokaryotic species.

引用

页码：289 / 296

页数：8

共 33 条

[1]

[Anonymous], 2007, COMPENDIUM SOIL FUNG, DOI DOI 10.1111/J.1365-2389.2008.01052_1.X

[2] CLASSIFICATION OF IMAGE DATA IN CONJUGATE REPRESENTATION SPACES [J].