SPECIFIC HYBRIDIZATION PROBES FOR MOUSE TYPE-I, TYPE-II, TYPE-III AND TYPE-IX COLLAGEN MESSENGER-RNAS

被引：152

作者：

METSARANTA, M

TOMAN, D

DECROMBRUGGHE, B

VUORIO, E

机构：

[1] UNIV TURKU,DEPT MED BIOCHEM,KIINAMYLLYNKATU 10,SF-20520 TURKU 52,FINLAND

[2] UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT MOLEC GENET,HOUSTON,TX 77030

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1089卷 / 02期

基金：

芬兰科学院; 美国国家卫生研究院; 英国医学研究理事会;

关键词：

COLLAGEN; GENE EXPRESSION; MESSENGER RNA; RECOMBINANT DNA; (MOUSE);

D O I：

10.1016/0167-4781(91)90014-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed DNA probes for the specific detection of mouse pro-alpha-1(I), pro-alpha-1(II), pro-alpha-1(III) and alpha-1(IX) collagen transcripts. To avoid cross-hybridization the probes for fibrillar collagens cover mainly sequences in the 3' untranslated region of the gene. Sequencing and Northern analysis confirmed that the clones share minimal sequence similarity and detect only the specific mRNAs under normal hybridization and washing conditions. The clone for mouse alpha-1(IX) collagen covers coding sequences but is sufficiently divergent from other collagen transcripts to allow specific detection of the corresponding mRNA.

引用

收藏

页码：241 / 243

页数：3

相关论文

共 14 条

[1] STRUCTURE OF CDNA CLONES CODING FOR THE ENTIRE PREPRO-ALPHA-1(III) CHAIN OF HUMAN TYPE-III PROCOLLAGEN - DIFFERENCES IN PROTEIN-STRUCTURE FROM TYPE-I PROCOLLAGEN AND CONSERVATION OF CODON PREFERENCES [J].

ALAKOKKO, L ;

KONTUSAARI, S ;

BALDWIN, CT ;

KUIVANIEMI, H ;

PROCKOP, DJ .

BIOCHEMICAL JOURNAL, 1989, 260 (02) :509-516

[2] NUCLEOTIDE-SEQUENCES OF COMPLEMENTARY DEOXYRIBONUCLEIC ACIDS FOR THE PRO-ALPHA-1 CHAIN OF HUMAN TYPE-I PROCOLLAGEN - STATISTICAL EVALUATION OF STRUCTURES THAT ARE CONSERVED DURING EVOLUTION [J].

BERNARD, MP ;

CHU, ML ;

MYERS, JC ;

RAMIREZ, F ;

EIKENBERRY, EF ;

PROCKOP, DJ .

BIOCHEMISTRY, 1983, 22 (22) :5213-5223

[3] DETERMINATION OF THE SINGLE POLYADENYLATION SITE OF THE HUMAN PRO-ALPHA-1(II) COLLAGEN GENE [J].

ELIMA, K ;

VUORIO, T ;

VUORIO, E .

NUCLEIC ACIDS RESEARCH, 1987, 15 (22) :9499-9504

[4] SEQUENCE DETERMINATION AND ANALYSIS OF THE 3' REGION OF CHICKEN PRO-ALPHA-1(I) AND PRO-ALPHA-2(I) COLLAGEN MESSENGER RIBONUCLEIC-ACIDS INCLUDING THE CARBOXY-TERMINAL PROPEPTIDE SEQUENCES [J].

FULLER, F ;

BOEDTKER, H .

BIOCHEMISTRY, 1981, 20 (04) :996-1006

[5] MOLECULAR-CLONING OF RAT AND HUMAN TYPE-IX COLLAGEN CDNA AND LOCALIZATION OF THE ALPHA-1(IX) GENE ON THE HUMAN CHROMOSOME-6 [J].

KIMURA, T ;

MATTEI, MG ;

STEVENS, JW ;

GOLDRING, MB ;

NINOMIYA, Y ;

OLSEN, BR .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 179 (01) :71-78

[6]

KUHN K, 1987, STRUCTURE FUNCTION C, P1

[7] A DISTINCT CLASS OF VERTEBRATE COLLAGEN GENES ENCODES CHICKEN TYPE-IX COLLAGEN POLYPEPTIDES [J].

LOZANO, G ;

NINOMIYA, Y ;

THOMPSON, H ;

OLSEN, BR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) :4050-4054

[8] 2 MESSENGER-RNAS OF MOUSE PRO ALPHA-1(I) COLLAGEN GENE DIFFER IN THE SIZE OF THE 3'-UNTRANSLATED REGION [J].

MOOSLEHNER, K ;

HARBERS, K .

NUCLEIC ACIDS RESEARCH, 1988, 16 (02) :773-773

[9] THE COMPLETE PRIMARY STRUCTURE OF 2 DISTINCT FORMS OF HUMAN ALPHA-1(IX) COLLAGEN CHAINS [J].

MURAGAKI, Y ;

KIMURA, T ;

NINOMIYA, Y ;

OLSEN, BR .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 192 (03) :703-708

[10] SYNTHESIS AND CHARACTERIZATION OF CDNA-ENCODING A CARTILAGE-SPECIFIC SHORT COLLAGEN [J].

NINOMIYA, Y ;

OLSEN, BR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (10) :3014-3018