PHENOTYPIC ANALYSIS OF B-CELLS EXTRACTED FROM HUMAN PERIODONTAL-DISEASE TISSUE

被引：13

作者：

GEMMELL, E

SEYMOUR, GJ

机构：

[1] Oral Biology Immunopathology Unit, Department of Dentistry, University of Queensland

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 06期

关键词：

PHENOTYPE; B-CELL; PERIODONTAL DISEASE;

D O I：

10.1111/j.1399-302X.1991.tb00507.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

B-cells extracted from periodontal disease tissue were analyzed for the presence of activation markers using a range of monoclonal antibodies. In adult periodontitis (AP), 6% of B-cells expressed the IL-2 receptor (CD25) compared with 1-2% in peripheral blood and healthy or marginal gingivitis (H/MG) gingival B-cells. There was also an increase in the mean percentage of IgD-positive B-cells and a decrease in CD21 and CD22 expression. In both AP and H/MG lesions, 20-22% of the B-cells expressed CD23 compared with < 5% in peripheral blood. As B-cells are activated by day 3 in culture and start differentiating into immunoglobulin-secreting cells by day 6, B-cell phenotypes were assayed at these times in this study. Following stimulation with the periodontopathic bacterium Porphyromonas gingivalis, the expression of CD23, CD21 and CD22 on B-cells extracted from AP lesions remained relatively constant over the 6-d culture period. However, with Fusobacterium nucleatum stimulation, there was a significant decrease in CD23, CD21 and CD22 expression after 3 d in culture, which corresponds to the activation time for B-cells. These results show that B-cells extracted from periodontal disease tissue display a range of activation markers and on stimulation, demonstrate differing responses to individual periodontopathic bacteria.

引用

页码：356 / 362

页数：7

共 39 条

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