P-GLYCOPROTEIN EXPRESSION IN NORMAL AND REACTIVE BONE MARROWS

被引:15
作者
HEGEWISCHBECKER, S
FLIEGNER, M
TSURUO, T
ZANDER, A
ZELLER, W
HOSSFELD, DK
机构
[1] UNIV HAMBURG, DEPT ONCOL & HAEMATOL, MED CLIN HAMBURG, W-2000 HAMBURG 20, GERMANY
[2] UNIV TOKYO, INST APPL MICROBIOL, TOKYO 113, JAPAN
关键词
D O I
10.1038/bjc.1993.83
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The expression of mdr1 gene product P-glycoprotein (P-gp) was investigated in 53 normal and reactive bone marrows by means of immunocytochemistry, using the monoclonal antibody (mAb) C219 and the alkaline phosphatase anti-alkaline phosphatase method. In a limited number of patients, data were confirmed by using the mAb MRK16 or a polymerase chain reaction assay for mdr1 gene expression. There was no history of prior chemotherapy or any malignancy in this group. Bone marrow aspirates were obtained as part of a routine diagnostic programme in bone marrow donors or in patients presenting with a variety of diagnoses such as unexplained gammopathy, fever, anaemia, other changes in peripheral blood smear, rheumatoid arthritis, vasculitis, or urticaria pigmentosa. Morphologically the bone marrow was normal in 23 patients, a megaloblastic erythropoiesis was seen in two patients and unspecific changes were seen in 28 patients. Twenty-seven of 53 samples were found to be positive for P-gp expression with the percentage of positive cells ranging from 2% - 80% (mean = 24%). With a cutoff point of 10%, five of 23 normal (22%) and 13 of 28 reactive bone marrows (46%) were considered positive for P-gp expression. There was no obvious correlation between diagnosis or age and P-gp expression. Additional staining for the early surface marker CD-34 was performed in 12 samples, with none of them revealing more than 1% positivity. Since P-gp expression has so far been described only in CD-34 positive bone marrow cells, data suggest that P-gp expression may be reinduced in CD-34 negative cells under conditions which remain to be determined.
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页码:430 / 435
页数:6
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