PLA2 ACTIVITY IN RAT-LIVER NUCLEI

被引：3

作者：

VANDERKLIS, FRM

WIERSINGA, WM

机构：

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 04期

关键词：

D O I：

10.1016/0020-711X(93)90666-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Subcellular fractions of rat liver were assayed for PLA2 activity. 2. The PLA, assay measures the release of [H-3]oleic acid from phospholipids, using labeled E. coli as substrate. 3. Nuclear fractions contained PLA2 activity, which was Ca2+ dependent and could not be explained from mitochondrial, microsomal or plasma membrane contamination. 4. The V(max) value of nuclear PLA2 is 0.30+/-0.04 pmol oleic acid/min/mg protein; its K(m) value is 0.86+/-0.12 muM, similar to that of mitochondrial PLA2. 5. We conclude that rat liver nuclei contain PLA2 activity.

引用

页码：575 / 579

页数：5

共 22 条

[1]

AARSMAN AJ, 1989, J BIOL CHEM, V264, P10008

[2]

APPEL W, 1974, METHOD ENZYMAT AN, V2, P958

[3] UPTAKE AND PHOSPHORYLATION OF PHOSPHATIDYLINOSITOL BY RAT-LIVER NUCLEI - ROLE OF PHOSPHATIDYLINOSITOL TRANSFER PROTEIN [J].