TUMOR-NECROSIS-FACTOR-ALPHA INDUCES P53-DEPENDENT APOPTOSIS IN RAT GLIOMA-CELLS

被引：39

作者：

YIN, DL

KONDO, S

BARNETT, GH

MORIMURA, T

TAKEUCHI, J

机构：

[1] CLEVELAND CLIN FDN,DEPT NEUROSURG,CTR BRAIN TUMOR,CTR CANC,CLEVELAND,OH 44195

[2] NATL UTANO HOSP,DEPT NEUROSURG,KYOTO,JAPAN

来源：

NEUROSURGERY | 1995年 / 37卷 / 04期

关键词：

APOPTOSIS; GLIOMA; MUTANT P53; TUMOR NECROSIS FACTOR-ALPHA; WILD-TYPE P53;

D O I：

10.1227/00006123-199510000-00021

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

IN THIS STUDY, we demonstrated that tumor necrosis factor (TNF)-alpha inhibited the viability of rat glioma (C6) cells and induced apoptosis but did not affect the viability of rat newborn brain, mainly astroglial cells. The antitumor activity of TNF-alpha against C6 cells was partially inhibited by actinomycin D and cycloheximide, suggesting that it is possibly dependent upon new ribonucleic acid and protein synthesis. The results of immunoblotting assay demonstrated that TNF-alpha decreased the expression of mutant p53 protein but induced the expression of wild-type p53 in C6 cells during apoptosis. We suggest that TNF-alpha may activate the function of wild-type p53 protein by the suppression of mutant p53, at least indirectly, and induce p53-dependent apoptosis in glioma cells.

引用

页码：758 / 762

页数：5

共 47 条

[1] MDM2 EXPRESSION IS INDUCED BY WILD TYPE-P53 ACTIVITY [J].