SEPARATION OF RELAXIN ACTIVITIES IN EXTRACTS OF OVARIES OF PREGNANT SOWS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引:8
作者
OLIVER, RM [1 ]
FIELDS, PA [1 ]
LARKIN, LH [1 ]
机构
[1] UNIV FLORIDA,COLL MED,JHMHC,GAINESVILLE,FL 32610
关键词
D O I
10.1677/joe.0.0760517
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Polyacrylamide gel electrophoresis has been employed to separate three fractions that have relaxin activity from crude extracts of ovaries of pregnant sows. Bioassays indicated that all fractions had the ability to inhibit spontaneous uterine contractions in vitro and to induce formation of interpubic ligaments in oestrogen-primed mice. The presence of the various fractions in crude extracts of sow ovaries was monitored with the polyacrylamide gel system on days 40, 70 and 100 of pregnancy. Both the total amount and the relative proportions of the fractions were found to change as pregnancy progressed.
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收藏
页码:517 / 525
页数:9
相关论文
共 17 条
[1]   RELAXIN IN PORCINE CORPORA-LUTEA DURING PREGNANCY AND AFTER HYSTERECTOMY [J].
ANDERSON, LL ;
FORD, JJ ;
MELAMPY, RM ;
COX, DF .
AMERICAN JOURNAL OF PHYSIOLOGY, 1973, 225 (05) :1215-1219
[2]   SPECIFICITY OF A RADIOIMMUNOASSAY FOR RELAXIN [J].
BRYANT, GD ;
STELMASIAK, T .
ENDOCRINE RESEARCH COMMUNICATIONS, 1974, 1 (5-6) :415-433
[3]   RELAXIN - STUDIES DEALING WITH ISOLATION, PURIFICATION, AND CHARACTERIZATION [J].
COHEN, H .
TRANSACTIONS OF THE NEW YORK ACADEMY OF SCIENCES, 1963, 25 (03) :313-&
[4]  
FINNEY DJ, 1952, STATISTICAL METHODS, P145
[5]  
FRIEDEN EH, 1953, RECENT PROG HORM RES, V8, P333
[6]  
FRIEDEN EH, 1974, FED PROC, V33, P1358
[7]   PURIFICATION AND ELECTROPHORETIC PROPERTIES OF RELAXIN PREPARATIONS [J].
FRIEDEN, EH .
TRANSACTIONS OF THE NEW YORK ACADEMY OF SCIENCES, 1963, 25 (03) :331-&
[8]  
FRIEDEN EH, 1977, P SOC EXP BIOL MED, V154, P407
[9]  
FRIEDEN EH, 1956, J BIOLOGICAL CHEMIST, V222, P616
[10]   ISOLATION AND PURIFICATION OF AN OVARIAN POLYPEPTIDE WITH UTERINE-RELAXING ACTIVITY [J].
GRISS, G ;
KECK, J ;
ENGELHORN, R ;
TUPPY, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 140 (01) :45-+