A PRELIMINARY STUDY OF NUCLEOPHILIC COMPETITION IN BETA-GALACTOSIDASE CATALYZED REACTIONS

被引:39
作者
VIRATELLE, O
TENU, JP
GARNIER, J
YON, J
机构
[1] Laboratoire d'Enzymologie Physico-chimique
关键词
D O I
10.1016/0006-291X(69)90236-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Evidence is given for the formation of an intermediary complex following the Michaelis complex in galactoside hydrolysis catalyzed by β-galactosidase. A two step scheme is proposed. The formation of the intermediary complex is the limiting step in phenylgalactoside hydrolysis, while each of the two steps is partially rate controlling in o-nitrophenylgalactoside hydrolysis. © 1969.
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页码:1036 / +
页数:1
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