HISTOCHEMICAL DETECTION OF CARBONIC-ANHYDRASE WITH DIMETHYLAMINONAPHTHALENE-5-SULFONAMIDE

被引：24

作者：

POCHHAMMER, C

DIETSCH, P

SIEGMUND, PR

机构：

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 07期

关键词：

D O I：

10.1177/27.7.112177

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A new specific method for the detection of carbonic anhydrase, EC 4.2.1.1, in tissues is described. The reaction of carbonic anhydrase with dimethylaminonaphthalene-5-sulfonamide (DNSA) forms a highly fluorescent complex. The specificity of the method is proved by the quenching of this fluorescence with ethoxzolamide (6-ethoxybenzothiazole-5-sulfonamide). The difference in the wavelength makes it possible to absorb the fluorescence of the unbound dimethylaminonaphthalene-5-sulfonamide by filters. Kidney, proventriculus, and bone from chicken have been examined. Carbonic anhydrase has been detected in the cytoplasm of the columnar lining cells, proximal tubule cells, and osteoclasts.

引用

页码：1103 / 1107

页数：5

共 15 条

[1]

CHEN RF, 1967, J BIOL CHEM, V242, P5813

[2]

DIETSCH P, 1978, ENDOCRINOLOGY CALCIU, P412

[3] CARBONIC-ANHYDRASE AND OSTEOCLASTS - LOCALIZATION BY LABELED INHIBITOR AUTORADIOGRAPHY [J].