ESTIMATION OF MEMBRANE-POTENTIALS OF INDIVIDUAL LYMPHOCYTES BY FLOW CYTOMETRY

被引：155

作者：

SHAPIRO, HM ^{[1
]}

NATALE, PJ ^{[1
]}

KAMENTSKY, LA ^{[1
]}

机构：

[1] ORTHO INSTRUMENTS, WESTWOOD, MA 02090 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 11期

关键词：

D O I：

10.1073/pnas.76.11.5728

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The membrane potentials of individual cells can be estimated by flow cytometric quantitation of the cells' uptake of the fluorescent lipophilic cationic dye 3,3'-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients are uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarizie only a fraction of the lymphocytes. The flow cytometric technique allows precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.

引用

页码：5728 / 5730

页数：3

共 14 条

[1] HYPERPOLARIZATION OF THYROID-CELLS INVITRO BY THYROTROPIN AND CYCLIC-AMP [J].