WNT-3, A GENE ACTIVATED BY PROVIRAL INSERTION IN MOUSE MAMMARY-TUMORS, IS HOMOLOGOUS TO INT-1/WNT-1 AND IS NORMALLY EXPRESSED IN MOUSE EMBRYOS AND ADULT BRAIN

被引：186

作者：

ROELINK, H ^{[1
]}

WAGENAAR, E ^{[1
]}

DASILVA, SL ^{[1
]}

NUSSE, R ^{[1
]}

机构：

[1] NETHERLANDS CANC INST,DIV MOLEC BIOL,1066 CX AMSTERDAM,NETHERLANDS

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 12期

关键词：

embryogenesis; int-1/Wnt-1 gene family; provirus tagging;

D O I：

10.1073/pnas.87.12.4519

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated a common insertion site, Wnt-3, for proviruses of the mouse mammary tumor virus (MMTV). Of mammary tumors induced by the GR variant of MMTV, 5% contains a provirus at Wnt-3, which is located on mouse chromosome 11. The gene is transcribed into a 3.8-kilobase (kb) mRNA in tumors with nearby proviral insertions but not in tumors with proviruses at other loci or in most adult tissues. Normal expression of Wnt-3 is detected in mouse embryos (with a peak around day 12 of gestation) and at low levels in adult brain. The transcriptional unit of the Wnt-3 gene spans ~ 55 kb, with a first intron of 36 kb. The deduced amino acid sequence of the Wnt-3 protein is 47% identical to the int-1/Wnt-1 gene product.

引用

页码：4519 / 4523

页数：5

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