LIGHT-SCATTERING TURBIDITY CHANGES AS A MEASURE OF THE KINETICS OF CA2+-PROMOTED AGGREGATION OF CHROMAFFIN GRANULE MEMBRANE GHOSTS

被引：22

作者：

MORRIS, SJ ^{[1
]}

HELLWEG, MA ^{[1
]}

HAYNES, DH ^{[1
]}

机构：

[1] UNIV MIAMI,SCH MED,DEPT PHARMACOL,MIAMI,FL 33152

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 553卷 / 02期

关键词：

(Kinetics; Membrane ghost); Ca[!sup]2+[!/sup]-promoted aggregation; Chromaffin granule; Turbidity change;

D O I：

10.1016/0005-2736(79)90237-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Changes in turbidity seen when chromaffin granule membrane ghosts are aggregated by Ca2+ can be modelled as dimerization of hollow spheres using Rayleigh-Gans-Debye light-scattering theory. The experimental changes agree well with the calculations. Thus, if shape or refractive index changes produced by osmotic perturbation, ion uptake, etc. can be excluded, turbidity readings can be used to follow the progress of the aggregation reaction of storage vesicles and other small particles or macromolecules. © 1979.

引用

页码：342 / 350

页数：9

共 27 条

[1] ADENOSINE TRIPHOSPHATE-EVOKED CATECHOLAMINE RELEASE IN CHROMAFFIN GRANULES - OSMOTIC LYSIS AS A CONSEQUENCE OF PROTON TRANSLOCATION [J].