STIMULATION OF CTP - PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE AND PHOSPHATIDYLCHOLINE SYNTHESIS BY INCUBATION OF RAT HEPATOCYTES WITH PHOSPHOLIPASE-A2

The effect of phospholipase A2 treatment of rat hepatocytes on CTP: phosphocholine cytidylyltransferase and phosphatidylcholine synthesis was investigated. Cytidylyltransferase is recovered from the cytosol and in a membranebound form with the microsomes. Digitonin treatment of cells causes rapid release into the medium of the cytosolic, but not the microsomal form of the cytidylyltransferase. Incubation of hepatocytes for 10 min with phospholipase A2 (0.9 units /dish) in the medium, resulted in a 33% decrease in the cytidylyltransferase activity released by digitonin treatment (2.5 ± 0.15 nmol/min per mg compared to 3.9 ± 0.10 nmol/min per mg in the control). In agreement with the digitonin experiments, incubation with 0.9 units /dish of phospholipase A2 resulted in a decrease in the cytidylyltransferase activity in the cytosol (from 4.3 ± 0.10 nmol/min per mg to 2.6 ± 0.14 nmol/min per mg) and a corresponding increase in the microsomal fraction (from 0.9 ± 0.16 nmol/min per mg to 1.8 ± 0.20 nmol/min per mg). The effect of phospholipase A2 on cytidylyltransferase translocation was concentration- and time-dependent. Incubation of hepaotcytes in the presence of phospholipase A2 (0.9 units/dish) for 10 min prior to pulse-chase experiments resulted in an increase in radiolabel incorporation into phosphatidylcholine (from 2.4 ± 0.02· 10-5 dpm/dish to 3.1 ± 0.1 · 10-5 dpm/dish) and a corresponding decrease in radiolabel associated with the choline (from 2.5 ± 0.05 · 10-5 to 1.4 ± 0.03 · 10-5 dpm) and phosphocholine fractions (from 8.5 ± 0.07 · 10-5 to 6.9 ± 0.05 · 10-5 dpm). We conclude that phospholipase A2 can cause a stimulation of CTP: phosphocholine cytidylyltransferase activity and phosphatidylcholine synthesis in cultured rat hepatocytes. © 1990.