ISOLATION OF A GENOMIC CLONE ENCODING THE RAT HISTONE VARIANT, H1D

被引：30

作者：

COLE, KD ^{[1
]}

KANDALA, JC ^{[1
]}

KREMER, E ^{[1
]}

KISTLER, WS ^{[1
]}

机构：

[1] UNIV S CAROLINA,DEPT CHEM,COLUMBIA,SC 29208

来源：

GENE | 1990年 / 89卷 / 02期

关键词：

chromosomal protein; nucleosomes; phage; λCharon; 4A; Recombinant DNA;

D O I：

10.1016/0378-1119(90)90015-J

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Mammals contain a family of five closely related H1 histone variants (H1a-e) as well as two less closely related forms, H1° and H1t. We have sequenced a rat genomic clone that encodes one of the standard H1 variants. An RNA transcript of the gene was made with bacteriophage SP6 RNA polymerase and translated in a cell-free system. The protein synthesized in vitro was identified as variant H1d by its electrophoretic mobility. © 1990.

引用

页码：265 / 269

页数：5

共 34 条

[1] TRANSCRIPTION TERMINATION AND 3' PROCESSING - THE END IS IN SITE [J].