DNA POLYMORPHISMS AMPLIFIED BY ARBITRARY PRIMERS ARE USEFUL AS GENETIC-MARKERS

被引：9484

作者：

WILLIAMS, JGK ^{[1
]}

KUBELIK, AR ^{[1
]}

LIVAK, KJ ^{[1
]}

RAFALSKI, JA ^{[1
]}

TINGEY, SV ^{[1
]}

机构：

[1] DUPONT CO,DEPT AGR PROD,WILMINGTON,DE 19880

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 22期

关键词：

D O I：

10.1093/nar/18.22.6531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendellan fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA. © 1990 Oxford University Press.

引用

页码：6531 / 6535

页数：5

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