HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF 1,2,3,4-TETRAHYDROISOQUINOLINE IN RAT-BRAIN WITH FLUORESCENCE DETECTION

被引：14

作者：

ISHIDA, J ^{[1
]}

YAMAGUCHI, M ^{[1
]}

NAKAMURA, M ^{[1
]}

机构：

[1] FUKUOKA UNIV,FAC PHARMACEUT SCI,NANAKUMA,JOHNAN KU,FUKUOKA 81401,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90313-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the fluorometric determination of 1,2,3,4-tetrahydroisoquinoline in rat brain is described. 1,2,3,4-Tetrahydroisoquinoline and 4-phenylpiperidine (internal standard) are isolated by liquid-liquid extraction, and then converted into the corresponding fluorescent derivatives with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives are separated within 60 min on a reversed-phase column, TSK gel ODS-120T, with isocratic elution, and detected fluorometrically. The detection limit of 1,2,3,4-tetrahydroisoquinoline is 1.0 pmol/g in rat brain ( S N = 3). © 1991.

引用

页码：168 / 171

页数：4

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