PRESENCE OF C-TYPE NATRIURETIC PEPTIDE IN CULTURED HUMAN ENDOTHELIAL-CELLS AND PLASMA

被引:250
作者
STINGO, AJ
CLAVELL, AL
HEUBLEIN, DM
WEI, CM
PITTELKOW, MR
BURNETT, JC
机构
[1] MAYO CLIN & MAYO FDN, DIV NEPHROL, ROCHESTER, MN 55905 USA
[2] MAYO CLIN & MAYO FDN, DIV DERMATOL, ROCHESTER, MN 55905 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 04期
关键词
NATRIURETIC PEPTIDES; ENDOTHELIUM; VASCULAR TONE; IMMUNOHISTOCHEMISTRY; RADIOIMMUNOASSAY;
D O I
10.1152/ajpheart.1992.263.4.H1318
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The present study was undertaken to investigate the presence of C-type natriuretic peptide (CNP) immunoreactivity in cultured human vascular endothelial cells and in human plasma. CNP immunoreactivity was present in cultured human aortic endothelial cells by both immunohistochemical staining and by radioimmunoassay. With the utilization of gel permeation chromatography, this immunoreactivity proved to be consistent with the higher molecular weight CNP-53. CNP immunoreactivity was also present in human plasma (n = 22) at low picogram concentrations (6.5 +/- 0.2 pg/ml) by specific radioimmunoassay. This immunoreactivity was consistent with the lower molecular weight CNP-22 by gel permeation chromatography. These findings suggest that the vascular endothelium may be the site of CNP production. The isolation of different molecular forms of CNP in tissue and plasma may be consistent with a storage form of the peptide in endothelial cells CNP-53, while CNP-22 circulates in plasma. In summary, the present study is consistent with CNP being a peptide of endothelial cell origin.
引用
收藏
页码:H1318 / H1321
页数:4
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