MULTICOMPONENT ANALYSIS OF HIGHLY OVERLAPPED HPLC PEAKS USING MULTIWAVELENGTH DIODE-ARRAY DETECTION

被引：23

作者：

CLADERA, A

GOMEZ, E

ESTELA, JM

CERDA, V

机构：

[1] Department of Chemistry, University of the Balearic Islands, Palma de Mallorca

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1992年 / 30卷 / 11期

关键词：

D O I：

10.1093/chromsci/30.11.453

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for the treatment of high-performance liquid chromatography (HPLC) data allowing the resolution of extensively overlapped peaks was developed. The method relies on the use of a diode array spectrophotometer and treatment of the generated data by least-squares linear regression. The applicability of the proposed method was checked on phenol mixtures. Because of the nature of the computational procedure, the results obtained depend neither on retention times nor on the shape or degree of overlap of the chromatographic peaks. The chief limitations of the proposed method are high degrees of spectral overlap and the need to know every co-eluting component. © 1992, Oxford University Press.

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页码：453 / 458

页数：6

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