APPLICATION OF HYDROPHOBIC INTERACTION CHROMATOGRAPHY (HIC) IN WATER ANALYSIS

被引：7

作者：

FUCHS, F

HEIDT, A

机构：

[1] Forschungsgruppe Sontheimer an der DVGW-Forschungsstelle, Engler-Bunte-Institut, Universität Karlsruhe, Karlsruhe, D-76128

来源：

ACTA HYDROCHIMICA ET HYDROBIOLOGICA | 1994年 / 22卷 / 03期

关键词：

HYDROPHOBIC INTERACTION CHROMATOGRAPHY; HIC; GEL FILTRATION CHROMATOGRAPHY; GFC; HUMIC SUBSTANCES; ADSORPTION ANALYSIS; OCTANOL WATER PARTITION COEFFICIENT;

D O I：

10.1002/aheh.19940220304

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Dissolved high molecular weight organic substances, like humic substances, can be characterized with regard to their molecular weight distribution using gel filtration chromatography (GFC). In addition, the adsorption properties, for example on activated carbon, can be described, if the substances are separated by hydrophobic interaction chromatography (HIC) in different fractions using a chromatographic column with a hydrophobic matrix. The retention time (Rt) of single chemical substances in this column can be used to characterize the hydrophobicity or hydrophility of each compound as well as of the high molecular weight organic matter. The hydrophobic interaction between the matrix of the column and the dissolved chemical compounds also depends on the hydrophobicity of the gel and the composition of the eluent. Relations between retention times, log P-coefficients (octanol/water partition) and K-values of isotherms on activated carbon (Freundlich equation) were found.

引用

页码：121 / 129

页数：9

共 25 条

[1]

Lehninger A., Biochemie, pp. 126-130, (1983)

[2]

Fuchs F., Gelchromatographische Trennung von organischen Wasserinhaltsstoffen, Teil I, II und III. Vom Wasser, 64, pp. 129-144, (1985)

[3]

Teil I, II und III. Vom Wasser, 65, pp. 93-105, (1985)

[4]

Teil I, II und III. Vom Wasser, 66, pp. 127-136, (1986)

[5]

Yamamoto S., Nomura M., Sano Y., Adsorption Chromatography of Proteins: Determination of Optimum Conditions, AIChE J., 33, pp. 1426-1434, (1987)

[6]

Kato Y., Kitamura T., Hashimoto T., High‐Performance Hydrophobic Interaction Chromatography of Proteins, J. Chromatogr., 266, pp. 49-54, (1983)

[7]

Pahlmann S., Rosengreen J., Hjerten S., Hydrophobic Interaction Chromatography on Uncharged Sepharose Derivates, Effects of Neutral Salts on the Adsorption of Proteins. J. Chromatogr., 131, pp. 99-108, (1977)

[8]

Aiken G.R., Isolation and Concentration Techniques for Aquatic Humic Substances, Humic Substances in Soil, Sediment, and Water, pp. 363-385, (1985)

[9]

Sontheimer H., Crittenden J.C., Summers R.S., Activated Carbon for Water Treatment, (1988)

[10]

Benz M., (1989)

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