FATTY-ACID ESTERIFICATION USING NYLON-IMMOBILIZED LIPASE

被引：67

作者：

ZAIDI, A ^{[1
]}

GAINER, JL ^{[1
]}

CARTA, G ^{[1
]}

机构：

[1] UNIV VIRGINIA,CTR BIOPROC TECHNOL,DEPT CHEM ENGN,CHARLOTTESVILLE,VA 22903

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 48卷 / 06期

关键词：

LIPASE; ENZYME IMMOBILIZATION; ESTERIFICATION; FATTY ACID; N-HEXANE;

D O I：

10.1002/bit.260480607

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The esterification of a long-chain fatty acid was conducted using a nylon-immobilized lipase from Candida cylindracea in a nearly anhydrous, nonpolar organic medium, hexane. Butyl laurate was produced from lauric acid and n-butanol at a maximum initial reaction rate of 37 mmol/h . g immobilized enzyme when the substrates were present in equimolar amounts at an initial concentration of 0.5 mol/L. Lower rates were obtained using nonstoichiometric amounts of the substrates. The rate of reaction increased with temperature, reaching a maximum between 35 and 45 degrees C and decreasing sharply at higher temperatures. (C) 1995 John Wiley & Sons, Inc.

引用

页码：601 / 605

页数：5

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