QUANTITATIVE SINGLE-CELL DETECTION OF POLY(BETA-HYDROXYBUTYRATE) ACCUMULATION IN RHIZOBIUM-MELILOTI BY FLOW-CYTOMETRY

被引：14

作者：

FOUCHET, P

JAN, S

COURTOIS, J

COURTOIS, B

FRELAT, G

BARBOTIN, JN

机构：

[1] UNIV PICARDIE,GENIE CELLULAIRE LAB,F-80039 AMIENS,FRANCE

[2] UNIV PICARDIE,BIOTECHNOL MICROBIENNE LAB,F-80025 AMIENS,FRANCE

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 126卷 / 01期

关键词：

POLY(BETA-HYDROXYBUTYRATE) ACCUMULATION; RHIZOBIUM MELILOTI; FLOW CYTOMETRY;

D O I：

10.1111/j.1574-6968.1995.tb07386.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ability of flow cytometry to detect intracellular accumulation of poly(beta-hydroxybutyrate) (PHB) in individual cells of Rhizobium meliloti was investigated using light scatter parameters. PHB accumulation during stationary phase was shown to form inclusion bodies inside cells which were detected by forward angle light scatter (FALS) parameter. A linear relationship was found between FALS intensity and percentage of PHB (w/w) of cells, demonstrating that flow cytometry allowed a quantitative analysis of intracellular PHB accumulation. In addition, results showed the ability of flow cytometry to quantify heterogeneity of rhizobia population in fermentation according to PHB production and accumulation.

引用

页码：31 / 35

页数：5

共 16 条

[1] ISOLATION AND CHARACTERIZATION OF CAROTENOID HYPERPRODUCING MUTANTS OF YEAST BY FLOW-CYTOMETRY AND CELL SORTING [J].