ESCHERICHIA-COLI ENDOTOXIN-MEDIATED ENDOTHELIAL INJURY IS MODULATED BY GLUTATHIONE ETHYL-ESTER

被引：11

作者：

MORRIS, PE ^{[1
]}

WHEELER, AP ^{[1
]}

MEYRICK, BO ^{[1
]}

BERNARD, GR ^{[1
]}

机构：

[1] VANDERBILT UNIV,DEPT PATHOL,DIV PULM & CRIT CARE,NASHVILLE,TN

来源：

JOURNAL OF INFECTIOUS DISEASES | 1995年 / 172卷 / 04期

关键词：

D O I：

10.1093/infdis/172.4.1119

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The mechanisms involved in endotoxin-induced endothelial injury are not fully understood, Oxidant stress is thought to play a role in cell damage after endotoxin exposure. Glutathione may ameliorate these affects. Glutathione ethyl ester (GSE) was used in bovine pulmonary artery endothelial cell (BPAEC) cultures to determine the potential for attenuation of endotoxin-induced injury. GSE (0.05-25 mM) was preincubated with BPAEC for 4 h before endotoxin exposure. Fresh media containing GSE and Escherichia coli endotoxin (0.05 mu g/mL) were then placed on the BPAEC and incubated for 18 h. GSE, at doses of 5 and 25 mM, attenuated endotoxin-induced injury, as reflected by a significant reduction in lactate dehydrogenase release. This was paralleled by a significant increase in endotoxin-stimulated prostaglandin E(2) and prostacyclin release. Thus, GSE attenuates endotoxin-induced injury of BPAEC in culture and alters BPAEC prostaglandin metabolism.

引用

页码：1119 / 1122

页数：4

共 13 条

[1] GLUTATHIONE MONOETHYL ESTER - PREPARATION, UPTAKE BY TISSUES, AND CONVERSION TO GLUTATHIONE [J].

ANDERSON, ME ;

POWRIE, F ;

PURI, RN ;

MEISTER, A .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 239 (02) :538-548

[2] ENDOTOXIN-MEDIATED ENDOTHELIAL-CELL INJURY AND ACTIVATION - ROLE OF SOLUBLE CD14 [J].

ARDITI, M ;

ZHOU, J ;

DORIO, R ;

RONG, GW ;

GOYERT, SM ;

KIM, KS .

INFECTION AND IMMUNITY, 1993, 61 (08) :3149-3156

[3] CONCENTRATION-ACTIVITY PROFILE OF THE MODULATION OF CYCLOOXYGENASE PRODUCT FORMATION BY REDUCED GLUTATHIONE IN MICROSOMAL FRACTIONS FROM THE GOAT LUNG [J].

BELLAN, JA ;

MINKES, RK ;

KERSTEIN, MD ;

SHAH, SV ;

KADOWITZ, PJ ;

CASSIN, S ;

MCNAMARA, DB .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1044 (03) :315-322

[4] HYPOXIA INCREASES THE SUSCEPTIBILITY OF PULMONARY-ARTERY ENDOTHELIAL-CELLS TO HYDROGEN-PEROXIDE INJURY [J].

BHAT, GB ;

TINSLEY, SB ;

TOLSON, JK ;

PATEL, JM ;

BLOCK, ER .

JOURNAL OF CELLULAR PHYSIOLOGY, 1992, 151 (02) :228-238

[5] ANTIOXIDANTS PROTECT CULTURED BOVINE LUNG ENDOTHELIAL-CELLS FROM INJURY BY ENDOTOXIN [J].

BRIGHAM, KL ;

MEYRICK, B ;

BERRY, LC ;

REPINE, JE .

JOURNAL OF APPLIED PHYSIOLOGY, 1987, 63 (02) :840-850

[6]

BUCKLEY BJ, 1991, J BIOL CHEM, V266, P16659

[7] CYTOPROTECTIVE EFFECT OF REDUCED GLUTATHIONE IN HYDROGEN PEROXIDE-INDUCED ENDOTHELIAL-CELL INJURY [J].

CHEN, SH ;

WU, HL ;

LIN, MT ;

JEN, CJ ;

WING, LYC ;

LEI, HY ;

TSAO, CJ ;

CHANG, WC .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1992, 45 (04) :299-305

[8]

DELMAESTRO RF, 1981, MICROVASC RES, V22, P225

[9] GLUTATHIONE [J].

MEISTER, A ;

ANDERSON, ME .

ANNUAL REVIEW OF BIOCHEMISTRY, 1983, 52 :711-760

[10]

MONTGOMERY AB, 1985, AM REV RESPIR DIS, V132, P485

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