SILICONE-BASED MICROCARRIERS - PREPARATION AND BHK CELL-CULTURE

被引：8

作者：

DENKBAS, EB

HOFFMAN, AS

PISKIN, E

机构：

[1] HACETTEPE UNIV,DEPT CHEM ENGN,ANKARA 06532,TURKEY

[2] HACETTEPE UNIV,DIV BIOENGN,ANKARA,TURKEY

[3] UNIV WASHINGTON,CTR BIOENGN,SEATTLE,WA

来源：

CHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL | 1995年 / 58卷 / 01期

关键词：

MICROCARRIERS; SILICONE; PREPARATION; BHK CELL CULTURE;

D O I：

10.1016/0923-0467(94)06094-0

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Spherical polymeric microbeads were produced by suspension cross-linking of hydroxyl-terminated polydimethylsiloxane prepolymers. Dimethylsiloxane-ethyleneoxide copolymer, dibutyltin dilaurate and methyltriethoxysilane were used as surfactant, catalyst and cross-linker, respectively. The surfaces of the microbeads were further modified by exposure of S-aminopropyltriethoxysilane plasma in a glow-discharge apparatus. These silicone-based microbeads were used as microcarriers in culturing of baby hamster kidney (BHK) cells both in stationary and in submerged culture conditions. High attachment and growth were achieved on the glow-discharge treated silicone microbeads with moderate surface wettabilities (contact angle: 43-46 degrees).

引用

页码：65 / 70

页数：6

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