MATING-BEHAVIOR OF PHYTOPHTHORA-PARASITICA - EVIDENCE FOR SEXUAL RECOMBINATION IN OOSPORES USING DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS AS GENETIC-MARKERS

被引:55
作者
FORSTER, H
COFFEY, MD
机构
[1] Department of Plant Pathology, University of California, Riverside
来源
EXPERIMENTAL MYCOLOGY | 1990年 / 14卷 / 04期
关键词
PHYTOPHTHORA-PARASITICA; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; NUCLEAR DNA; MITOCHONDRIAL DNA; SEXUAL RECOMBINATION;
D O I
10.1016/0147-5975(90)90058-2
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cloned nuclear DNA fragments that detected restriction fragment length polymorphisms (RFLPs) in homozygous loci of isolates of Phytophthora parasitica were used as genetic markers to investigate sexual recombination during oospore formation. It was found that the majority of the 23 oospore progeny studied in each of the two crosses carried both of the parental markers. However, aberrant recombination patterns were observed; some of the progeny were homozygous at one RFLP locus, whereas at another locus both of the parental markers were present. Only two of the progeny of each cross did not show sexual recombination with any of the four or five RFLP markers used. Mitochondrial DNA (mtDNA) was uniparentally inherited. In both crosses the majority of the progeny carried the mtDNA type of one of the common parental strains. © 1990 Academic Press, Inc.
引用
收藏
页码:351 / 359
页数:9
相关论文
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