USE OF CENTRIFUGAL PARTITION CHROMATOGRAPHY AND PROTEINS IN THE PREPARATIVE SEPARATION OF AMINO-ACID ENANTIOMERS

被引:13
作者
ARMSTRONG, DW
MENGES, R
WAINER, IW
机构
[1] Department of Chemistry, University of Missouri-Rolla, Rolla
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 18期
关键词
D O I
10.1080/01483919008049556
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The growth of analytical methodologies for the separation of enantiomers has been impressive. Attention is now turning to the large scale separation of enantiomers. Often scaling-up sensitive analytical separations is ineffective and inefficient. Centrifugal partition chromatography (CPC) may be a viable alternative for the preparative separation of racemic mixtures in some cases. The use of proteins as chiral selectors in CPC is examined. Attention was focused on proteins that previously were used as bonded phases in analytical LC columns. The enzymatic properties of alpha-chymotrypsin allowed it to be used as a bioreactor in conjunction with CPC. When proteins are used as components of the stationary or mobile phase there can be problems with denaturation. However, when used in external incubation processes or as column bioreactors coupled with CPC, effective gram-scale separations can be performed. Tryptophan methyl ester was used as a model compound to evaluate this approach.
引用
收藏
页码:3571 / 3581
页数:11
相关论文
共 13 条
[1]   DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .3. OPTICAL RESOLUTION OF A SERIES OF N-AROYL D,L-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN COVALENTLY BOUND TO SILICA [J].
ALLENMARK, S ;
BOMGREN, B ;
BOREN, H .
JOURNAL OF CHROMATOGRAPHY, 1983, 264 (01) :63-68
[2]   STUDY OF THE ORIGIN AND MECHANISM OF BAND BROADENING AND PRESSURE-DROP IN CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY [J].
ARMSTRONG, DW ;
BERTRAND, GL ;
BERTHOD, A .
ANALYTICAL CHEMISTRY, 1988, 60 (22) :2513-2519
[3]   OPTICAL ISOMER SEPARATION BY LIQUID-CHROMATOGRAPHY [J].
ARMSTRONG, DW .
ANALYTICAL CHEMISTRY, 1987, 59 (02) :A84-&
[4]   POLAR-LIQUID, DERIVATIZED CYCLODEXTRIN STATIONARY PHASES FOR THE CAPILLARY GAS-CHROMATOGRAPHY SEPARATION OF ENANTIOMERS [J].
ARMSTRONG, DW ;
LI, WY ;
CHANG, CD ;
PITHA, J .
ANALYTICAL CHEMISTRY, 1990, 62 (09) :914-923
[5]   CENTRIFUGAL PARTITION CHROMATOGRAPHY .4. PREPARATIVE SAMPLE PURIFICATION AND PARTITION-COEFFICIENT DETERMINATION [J].
BERTHOD, A ;
ARMSTRONG, DW .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (06) :1187-1204
[6]   CENTRIFUGAL PARTITION CHROMATOGRAPHY .2. SELECTIVITY AND EFFICIENCY [J].
BERTHOD, A ;
ARMSTRONG, DW .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (03) :567-583
[7]   CENTRIFUGAL PARTITION CHROMATOGRAPHY .1. GENERAL FEATURES [J].
BERTHOD, A ;
ARMSTRONG, DW .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (03) :547-566
[8]   DIRECT LIQUID-CHROMATOGRAPHIC RESOLUTION OF RACEMIC DRUGS USING ALPHA-1-ACID GLYCOPROTEIN AS THE CHIRAL STATIONARY PHASE [J].
HERMANSSON, J .
JOURNAL OF CHROMATOGRAPHY, 1983, 269 (02) :71-80
[9]  
JADUAD P, 1989, CHIRALITY, V1, P38
[10]  
JADUAD P, 1990, CHIRALITY, V2, P32