AN IMMUNOHISTOCHEMICAL STUDY OF THE PNEUMONIA CAUSED BY PESTE-DES-PETITS RUMINANTS VIRUS

被引:34
作者
BROWN, CC
MARINER, JC
OLANDER, HJ
机构
[1] UNIV CALIF DAVIS,DEPT VET PATHOL,DAVIS,CA 95616
[2] US SCI & TECHNOL SERV,GREENPORT,NY
[3] US ANIM & PLANT HLTH INSPECT SERV,GREENPORT,NY
关键词
GOAT; PESTE-DES-PETITS RUMINANTS VIRUS; PNEUMONIA; IMMUNOHISTOCHEMISTRY;
D O I
10.1177/030098589102800209
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Ten goats were inoculated with peste des petitis ruminants virus, a paramyxovirus closely related to rinderpest virus. All goats developed severe clinical disease, 8/10 having coughing or dyspnea as prominent clinical signs. In addition, all of the goats had stomatitis and diarrhea. Histopathologic and immunohistochemical studies were done only on the respiratory tracts. Pathologic changes ranged from mild multifocal bronchiolitis and bronchitis to severe bronchointerstitial pneumonia. Lesions were more severe in anteroventral than caudal lobes. The histologic nature of the viral process in the goat lungs had many features in common with the processes of pneumonia in dogs, due to canine distemper, or pneumonia in human beings, due to measles virus. Immunohistochemical staining of formalin-fixed, paraffin-embedded respiratory tract tissue was performed using an indirect system with rabbit anti-rinderpest virus serum, biotinylated anti-rabbit antibody, streptavidin-alkaline phosphatase, and nitroblue tetrazolium chromogen. Staining was sensitive, highlighting the presence of viral antigen in both lung and trachea of all goats. Viral antigen was found in both cytoplasm and nucleus of tracheal, bronchial, and bronchiolar epithelial cells, type II pneumocytes, syncytial cells, and alveolar macrophages. In general, the amount of staining correlated directly with the severity of the inflammatory process.
引用
收藏
页码:166 / 170
页数:5
相关论文
共 9 条
[1]  
BUNDZA A, 1988, CAN J VET RES, V52, P46
[2]  
Dungworth DC, 1985, PATHOLOGY DOMESTIC A, V3rd, P413
[3]  
GIBBS EPJ, 1979, INTERVIROLOGY, V22, P268
[4]  
ROBBINS SL, 1979, PATHOLOGIC BASIS DIS, P440
[5]   PATHOLOGY OF AN EROSIVE STOMATITIS AND ENTERITIS IN WEST-AFRICAN DWARF GOATS [J].
ROWLAND, AC ;
SCOTT, GR ;
HILL, DH .
JOURNAL OF PATHOLOGY, 1969, 98 (01) :83-&
[6]  
SHAILA MS, 1989, VET REC, V125, P602
[7]   SEROLOGICAL STUDIES WITH THE VIRUS OF PESTE DES PETITS RUMINANTS IN NIGERIA [J].
TAYLOR, WP .
RESEARCH IN VETERINARY SCIENCE, 1979, 26 (02) :236-242
[8]   THE DISTRIBUTION AND EPIDEMIOLOGY OF PESTE DES PETITS RUMINANTS [J].
TAYLOR, WP .
PREVENTIVE VETERINARY MEDICINE, 1984, 2 (1-4) :157-166
[9]   PROTECTION OF GOATS AGAINST PESTE-DES-PETITS-RUMINANTS WITH ATTENUATED RINDERPEST VIRUS [J].
TAYLOR, WP .
RESEARCH IN VETERINARY SCIENCE, 1979, 27 (03) :321-324