CHARACTERIZATION OF THE PROMOTER FROM THE SINGLE-COPY GENE ENCODING FERREDOXIN-NADP+-OXIDOREDUCTASE FROM SPINACH

被引:40
作者
OELMULLER, R
BOLLE, C
TYAGI, AK
NIEKRAWIETZ, N
BREIT, S
HERRMANN, RG
机构
[1] Botanisches Institut der Ludwig-Maximilians-Universität, München 19, W-8000
来源
MOLECULAR AND GENERAL GENETICS | 1993年 / 237卷 / 1-2期
关键词
CIS REGULATORY ELEMENTS; FERREDOXIN-NADP+-OXIDOREDUCTASE; LIGHT-RESPONSIVE ELEMENTS; P(FR)-INDUCED SIGNAL TRANSDUCTION; PROMOTER ANALYSIS;
D O I
10.1007/BF00282808
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We describe a genomic DNA segment from spinach that bears part of the single-copy gene for ferredoxin-NADP+-oxidoreductase (FNR) including a 3.4 kb promoter sequence. Dissection of this DNA segment and its analysis in GUS (beta-glucuronidase) gene fusions in transgenic tobacco demonstrated that the promoter differs in structure from all other promoters for thylakoid protein genes studied to date. Two regions with light-responsive elements were identified. One is located within the first 118 bp upstream of the transcription initiation site. A second fragment covering nucleotide positions -220 to -119 is capable of conferring light-dependent GUS gene expression on two different minimal promoters. The latter fragment binds a trans-acting factor in gel-shift assays. None of the fragments carries cis elements known from other genes to be involved in light-controlled expression. Comparison of the light responsiveness of GUS gene fusions controlled by the -753/+231 and -118/+231 regions indicates that they respond differentially to phytochrome-dependent signals and that their expression in tobacco is not restricted to tissue with functional chloroplasts.
引用
收藏
页码:261 / 272
页数:12
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