SEPARATION AND DETERMINATION OF CARBADOX, NITROFURAZONE, NITROFURANTOIN, FURAZOLIDONE, AND FURALTADONE IN THEIR MIXTURES BY THIN-LAYER AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The separation and quantitative determination of five drugs, namely carbadox, nitrofurazone, nitrofurantoin, furazolidone and furaltadone, in their various mixtures of 3, 4 or 5 components was investigated. Two types of mobile phases were examined for the TLC separation of the drugs, chloroform/ acetonitrile/formic acid and chloroform/acetone. Two other mobile phases were also examined for the high performance liquid chromatographic determination of them, acetonitrile/sodium acetate and acetonitrile/sodium dihydrogen phosphate. The resolution of the chromatograms was studied in both cases and also the regression lines of the quantitative determination were described. The absolute detection limits of the determination were in the range of 0.2-1.6 ng for the five compounds.