CAPILLARY ELECTROPHORESIS OF ISOQUINOLINE ALKALOIDS FROM CHELIDONIUM-MAJUS L

被引：14

作者：

PIETTA, PG

MAURI, PL

GARDANA, C

COLOMBO, ML

TOME, F

机构：

[1] CNR,IST TECNOL BIOMED AVANZATE,I-20131 MILAN,ITALY

[2] IST SCI FARMACOL,I-20133 MILAN,ITALY

[3] DIPARTIMENTO BIOL,I-20133 MILAN,ITALY

来源：

PHYTOCHEMICAL ANALYSIS | 1995年 / 6卷 / 04期

关键词：

CAPILLARY ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; DIODE-ARRAY DETECTION; CHELIDONIUM MAJUS; PAPAVERACEAE; ISOQUINOLINE ALKALOIDS;

D O I：

10.1002/pca.2800060404

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of six different isoquinoline alkaloids from Chelidonium majus (leaves and roots) using capillary electrophoresis is described. Analyses were performed on fused silica capillaries with 66 mM phosphate buffer at pH 2.4 containing 10% methanol. The analytes were monitored by on-line ultraviolet (UV) detection to identify sanguinarine, chelerythrine, berberine and chelidonine. Both coptisine and DL-stylopine were isolated by semi-preparative high performance liquid chromatography (HPLC), and their structure assessed by UV and mass spectrometry. Capillary electrophoresis is confirmed as a useful complementary technique to HPLC.

引用

页码：196 / 202

页数：7

共 18 条

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[2] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF QUATERNARY ALKALOIDS OF CHELIDONIUM-MAJUS L ROOTS [J].